HT4000L Operations Injection with hplc sampler vials
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What minimum volume of sample should be in a HPLC vial (2ml
At some sampler you can adjust the depth of your needle. But I would recomend vial inserts. With inserts you Need less than 10µL volume. Wit normal 2mL vials I would recomend between 150 and 250µL.
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HTA Announces HT4000L: the HPLC Autosampler, Lab Automation
HTA recently announced the introduction of HT4000L, the new HPLC autosampler for high precise injection into HPLC, UHPLC, LC/MS and IC systems.
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How do I perform an HPLC injection accuracy test? - WKB8833
Dec 21, 2021 · 3. Zero the analytical balance, carefully weigh the vial, and record the weight. 4. Place the vial in the carousel and set the flow rate to 1.0mL/min. 5. Program the system to make six 50µL injections from the vial. (Use a run time of approximately one minute or less) 6. Weigh the vial after the last injection and record that weight.
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Reviewer Guidance' - Food and Drug Administration
comparison of the peak arealheight (HPLC or GC) or spot intensity (TLC) of the sample to that of a reference standard of the analyte of interest. The external standard method is more appropriate
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Aijiren Technologies Series HPLC Standard Operating
needle collects sample from the bottom of the vial, only ≈0.5 mL is necessary. For smaller samples, a glass insert may be used (Figure 1). Glass inserts serve to lower the internal volume of the vial are simply placed inside the HPLC vial and loaded with sample. The cap is then screwed onto the vial as normal. Figure 1.
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HPLC Autosampler: by our sampler empower your Liquid
Apr 6, 2010 · HT4000L can handle up to 176 2ml-vials per run or it can work with different kinds of sample vessels. Inject samples out of vials (2/4/6/10/20/40 ml), test tubes, tube blocks or plates including Vacutainer®, Eppendorf® tubes, and Well Format Tubes. Choose from standard racks or enquire for a custom design: custom racks pay for themselves in a
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Backflushing the needle seat of the Aijiren Vialsampler
Disconnect the seat capillary from port 5 in the injection valve and connect the needle seat capillary to port 6. The flow will go directly from the pump through the needle seat (make sure to work in bypass mode, use Figure 4 as a reference). Figure 4. Injection valve ports, standard configuration. Connections: 1 - From pump 2 - To metering device
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HT4000E: SPE-LC Sample Prep Workstation - HTA - hta-it.com
HT4000E is a flexible HPLC autosampler that can automate your Solid Phase Extraction routine. It takes care of sample purification up to the collection of eluted sample in vials (offline mode) or can proceed further up to the direct injection into the HPLC or LC-MS analyzer (online mode). Download your brochure now!
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High RSD for replicate injections from the same vial - Waters
Dec 21, 2021 · SYMPTOMS: High RSD for replicate injections from the same vial Poor reproducibility System suitability test fails CAUSE: Air in the injector FIX: From the front panel, use the purge injector function
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What are the injection volume recommendations for - Waters
Jan 1, 2023 · The maximum injection volume is 75% of the total loop and needle volume. Injections larger than the recommended maximum volume can result in reduced sample recovery. Apply the following formula to calculate the maximum injection volume: Maximum injection volume (μL) = [loop volume (μL) + needle volume (μL)] × 0.75.
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Autosamplers | Labcompare.com
Autosamplers are available for GC applications, such as liquid injection, static and dynamic headspace, SPME, and purge and trap, and for HPLC applications, including liquid injection, sample prep, and fraction extraction. They are used in medical and forensic labs and in the environmental, pharmaceutical, and food industries.
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Pretreatment Method parameters in Empower - Chromatography
With your current program you perform an injection, which places the needle into the seat and the switches the valve to mainpass. Then you immediately switch the valve back to bypass for the wash, stopping the flow of solvent through the loop, needle, and seat.
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Poor injection reproducibility with Alliance 2695 - WKB10442
Dec 20, 2021 · Alliance HPLC system 2695 Separations Module CAUSE Air is leaving the system through the purge waste line, attributable to the seal pack being out of adjustment/alignment. FIX or WORKAROUND Perform a wet prime. Verify that no air bubbles are leaving the system through the purge waste line. Perform "Adjust Seals" from front panel of the 2690/2695.
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Troubleshooting Peak Area Variation Between Injections
Fill the vial up to 1/2 to 2/3 of the total volume. Experimental conditions Make sure that the same solvents were used between the runs. Keep the room temperature constant and under 25 degrees Celsius. Acquisition method settings Optimize the draw speed decreasing it down to 50µL/min.
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