Irrespective of residue identity, in-gel fluorescence analysis reports on the presence of HNE modification on the POIs. Residue specificity in POI modification is determined by LC–MS/MS analysis post cell lysis and resin-assisted enrichment (Fig. 5c). Once a specific sensor protein has been earmarked by T-REX, target- and residue(s)-specific
Wide Opening Purification Reagent Bottle Analysis Aldrich
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